Kaempferol Inhibits Dry Eye-Related Corneal Inflammation In Vitro Via the p38 Pathway

ObjectiveThe purpose of this study is to investigate the effect kaempferol (KAE), an effective constituent Buddleja officinalis, on human corneal epithelial cells (HCECs) under a hyperosmolar environment. Methods HCECs were cultured in DMEM/F12 medium and allocated into four groups: normal, model, vehicle, KAE. Ki67 staining was used detect HCEC proliferation; TdT-mediated dUTP Nick-End Labeling apoptosis; quantified real-time PCR mRNA relative expression levels TNF-α, IL-6, p38. Western blot reveal protein Results Compared with those normal group, proliferation ability significantly inhibited apoptosis rate notably increased model group; but KAE could effectively improve reduce apoptosis. expressions p38 which decreased after intervention. Conclusion promote cell apoptosis, as well inhibit inflammatory factors hypertonic-induced via p38MAPK pathway.